CAS No. 3520-42-1

Batch: Purity:99.03
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Sulforhodamine B sodium salt Chemical Structure
Chemical Structure
Check the Sulforhodamine B sodium salt product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name sodium 4-[3-(diethylamino)-6-diethylazaniumylidenexanthen-9-yl]benzene-1,3-disulfonate
CAS Number 3520-42-1
Molecular Formula
C27H29N2NaO7S2
Molecular Weight (MW) 580.65
SMILES [Na+].CCN(CC)C1=CC2=C(C=C1)C(=C3C=CC(C=C3O2)=[N+](CC)CC)C4=C(C=C(C=C4)[S]([O-])(=O)=O)[S]([O-])(=O)=O
InChIKey SXQCTESRRZBPHJ-UHFFFAOYSA-M

Biological Activity

Sulforhodamine B sodium salt is a protein-specific fluorescent dye widely used to quantify total cellular protein content, cell expansion, and proliferation. The dye binds stoichiometrically to basic amino acid residues under mild acidic conditions, providing an accurate colorimetric readout of cellular biomass. By measuring total protein mass rather than enzymatic activity, it serves as a robust tool for evaluating cell cytotoxicity and growth dynamics in high-throughput screening assays. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 100 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Skehan P, et al. J Natl Cancer Inst. 1990;82(13):1107-12.

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Note: Technical data last updated: Sep 1, 2026.