CAS No. 3118-97-6

Batch: Purity:98.24
Sudan II Chemical Structure
Chemical Structure
Check the Sudan II product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 3118-97-6
Molecular Formula

C18H16N2O

Molecular Weight (MW) 276.33
SMILES CC1=CC=C(N=NC2=C3C=CC=CC3=CC=C2O)C(=C1)C

Ordering Information

Biological Activity

Sudan II (Solvent Orange 7) is a lipophilic azo dye that selectively partitions into non-polar lipid droplets and lipoproteins. As a lysochrome dye, Sudan II passively diffuses across cell membranes and accumulates in lipid-rich structures, enabling the histological staining and visualization of intracellular triglycerides and lipid deposits. In cellular studies, exposure to Sudan II alters lipid membrane dynamics and induces oxidative stress and cytotoxic responses in treated cells.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 30 mg/mL (108.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 1 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.