CAS No. 70967-97-4
Batch:
Purity:98.03
Chemical Structure
Check the
Suc-Ala-Ala-Pro-Phe-pNA
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| CAS Number | 70967-97-4 | |
|---|---|---|
| Molecular Formula |
C30H36N6O9 |
|
| Molecular Weight (MW) | 624.65 |
Biological Activity
Suc-Ala-Ala-Pro-Phe-pNA (Suc-AAPF-pNA) is a chromogenic p-nitroanilide substrate for chymotrypsin-like serine proteases, including cathepsin G and chymotrypsin, exhibiting a Km of 1.7 mM. Proteolytic cleavage releases 4-nitroaniline, enabling spectrophotometric quantification of enzyme kinetics and screening of protease inhibitors. This enzymatic cleavage monitors serine protease catalytic activity without cross-reactivity to elastase, supporting functional characterization of neutrophil granule enzymes and proteolysis signaling pathways.[1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
120 mg/mL
(192.1 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 120 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.