CAS No. 22189-32-8

Batch: Purity:99.26
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Spectinomycin dihydrochloride pentahydrate Chemical Structure
Chemical Structure
Check the Spectinomycin dihydrochloride pentahydrate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 22189-32-8
Molecular Formula

C14H36Cl2N2O12
Molecular Weight (MW) 495.35
SMILES O.O.O.O.O.Cl.Cl.CNC1C(O)C(NC)C2OC3(O)C(OC(C)CC3=O)OC2C1O

Ordering Information

Biological Activity

Spectinomycin dihydrochloride pentahydrate is an aminocyclitol antibiotic that acts as a bacterial protein synthesis inhibitor by binding to the 30S ribosomal subunit. By binding to helix 34 of 16S rRNA, it disrupts ribosomal translocation of messenger RNA and transfer RNAs. This impairment of translation elongation inhibits bacterial cell growth across a broad spectrum of Gram-negative and Gram-positive pathogens. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 99 mg/mL (199.85 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 99 mg/mL
Ethanol 11 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.