CAS No. 54642-23-8

Batch: Purity:99.38
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SP 600125, negative control Chemical Structure
Chemical Structure
Check the SP 600125, negative control product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 14-methyl-14,15-diazatetracyclo[7.6.1.02,7.013,16]hexadeca-1(15),2,4,6,9(16),10,12-heptaen-8-one
CAS Number 54642-23-8
Molecular Formula

C15H10N2O
Molecular Weight (MW) 234.25
InChIKey ODZGYELAMAOARP-UHFFFAOYSA-N

Biological Activity

SP 600125, negative control is a methylated derivative that serves as an inactive control for the c-Jun N-terminal kinase (JNK) inhibitor SP600125, which inhibits JNK1, JNK2, and JNK3 (Ki 0.19 µM). While active SP600125 suppresses JNK pathway activity, this negative control analog fails to reduce cAMP-stimulated mRNA levels of downstream targets such as Aa-Nat, Period1, and MKP-1, helping rule out off-target effects in JNK signaling research. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 12 mg/mL (51.22 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Bennett BL, et al. Proc Natl Acad Sci U S A. 2001;98(24):13681-6.

[2] Chansard M, et al. Brain Res Mol Brain Res. 2005;139(2):333-40.

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Note: Technical data last updated: Sep 1, 2026.