CAS No. 438190-29-5

Batch: Purity:99.04
SMI-4a Chemical Structure
Chemical Structure
Check the SMI-4a product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (5Z)-5-[[3-(trifluoromethyl)phenyl]methylidene]-1,3-thiazolidine-2,4-dione
CAS Number 438190-29-5
Molecular Formula

C11H6F3NO2S

Molecular Weight (MW) 273.23
SMILES C1=CC(=CC(=C1)C(F)(F)F)C=C2C(=O)NC(=O)S2
InChIKey NGJLOFCOEOHFKQ-YVMONPNESA-N

Ordering Information

Biological Activity

SMI-4a is a potent small-molecule inhibitor targeting Pim-1 kinase with modest activity against Pim-2. By inhibiting PIM kinase activity, SMI-4a disrupts downstream mTOR and BAD signaling pathways, leading to G1-phase cell cycle arrest and induction of apoptosis in precursor-B acute lymphoblastic leukemia cells. Furthermore, SMI-4a treatment suppresses leukemic blast proliferation and synergizes with dexamethasone to enhance cytotoxic effects in hematologic malignancy models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 55 mg/mL (201.29 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 23 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.