CAS No. 67200-34-4
Batch:
Purity:99.02
Chemical Structure
Check the
SMER3
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 13-oxa-10,12,14,16-tetrazatetracyclo[7.7.0.02,7.011,15]hexadeca-1(16),2,4,6,9,11,14-heptaen-8-one | |
|---|---|---|
| CAS Number | 67200-34-4 | |
| Molecular Formula |
C11H4N4O2 |
|
| Molecular Weight (MW) | 224.18 | |
| InChIKey | SFSSAKVWCKFRHE-UHFFFAOYSA-N |
Biological Activity
SMER3 is a selective small-molecule inhibitor of the Skp1-Cullin-F-box (SCF) Met30 ubiquitin ligase with an IC50 of 51 nM. By inhibiting SCF Met30 ubiquitin ligase activity, SMER3 blocks target substrate ubiquitination and downstream protein turnover. This targeted inhibition modulates cellular nutrient-sensing pathways, ultimately enhancing rapamycin-mediated antiproliferative effects and growth inhibition in yeast cell models. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
15 mg/mL
(66.91 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.