CAS No. 1259484-97-3
Batch:
Purity:99.79
Chemical Structure
Check the
SKI-178
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | N-[(E)-1-(3,4-dimethoxyphenyl)ethylideneamino]-3-(4-methoxyphenyl)-1H-pyrazole-5-carboxamide | |
|---|---|---|
| CAS Number | 1259484-97-3 | |
| Molecular Formula |
C21H22N4O4 |
|
| Molecular Weight (MW) | 394.42 | |
| SMILES | COC1=CC=C(C=C1)C2=N[NH]C(=C2)C(=O)N\N=C(C)\C3=CC(=C(OC)C=C3)OC | |
| InChIKey | GMFUWEBOUKIKRP-LPYMAVHISA-N |
SKI-178 is a dual small-molecule inhibitor targeting sphingosine kinase 1 (SphK1) and sphingosine kinase 2 (SphK2). By inhibiting SphK enzymatic activity, SKI-178 disrupts intracellular sphingolipid signaling and suppresses pro-survival cascades. These downstream effects result in cell cycle arrest and induce sustained apoptotic cell death in human acute myeloid leukemia cells through a CDK1-dependent signaling mechanism.
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Concentration
Volume
Mass
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Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
79 mg/mL
(200.29 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.