CAS No. 127373-66-4

Batch: Purity:99.32
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Sivelestat (Ono-5046) Chemical Structure
Chemical Structure
Check the Sivelestat (Ono-5046) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-[[2-[[4-(2,2-dimethylpropanoyloxy)phenyl]sulfonylamino]benzoyl]amino]acetic acid
CAS Number 127373-66-4
Molecular Formula

C20H22N2O7S
Molecular Weight (MW) 434.46
SMILES CC(C)(C)C(=O)OC1=CC=C(C=C1)S(=O)(=O)NC2=CC=CC=C2C(=O)NCC(=O)O
InChIKey BTGNGJJLZOIYID-UHFFFAOYSA-N

Ordering Information

Biological Activity

Sivelestat (Ono-5046) is a potent and selective inhibitor of human neutrophil elastase (IC50 of. By inhibiting neutrophil elastase activity, it prevents extracellular matrix degradation and endothelial cell injury induced by activated neutrophils. Consequently, Sivelestat attenuates downstream inflammatory signaling pathways, suppressing inflammatory mediator release and tissue damage while displaying high selectivity over other serine proteases. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 87 mg/mL (200.24 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 8 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.