CAS No. 2146095-85-2
Batch:
Purity:99.05
Chemical Structure
Check the
SHIN1(RZ-2994)
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 6-amino-4-[3-(hydroxymethyl)-5-phenylphenyl]-3-methyl-4-propan-2-yl-2H-pyrano[2,3-c]pyrazole-5-carbonitrile | |
|---|---|---|
| CAS Number | 2146095-85-2 | |
| Molecular Formula |
C24H24N4O2 |
|
| Molecular Weight (MW) | 400.47 | |
| InChIKey | VVVOFJZXKJKHTD-UHFFFAOYSA-N |
SHIN1 (RZ-2994) is a potent dual inhibitor of human serine hydroxymethyltransferase 1 (SHMT1, IC50 = 5 nM) and SHMT2 (IC50 = 13 nM). By targeting SHMT1 and SHMT2, SHIN1 disrupts folate-mediated one-carbon metabolism, leading to a progressive reduction in intracellular purine nucleotide levels. This depletion impairs DNA synthesis and cellular proliferation, thereby effectively inhibiting the growth of susceptible cancer cells in vitro.
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Concentration
Volume
Mass
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Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
80 mg/mL
(199.76 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 5 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.