CAS No. 304909-07-7

Batch: Purity:99.03
SANT-1 Chemical Structure
Chemical Structure
Check the SANT-1 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (E)-N-(4-benzylpiperazin-1-yl)-1-(3,5-dimethyl-1-phenylpyrazol-4-yl)methanimine
CAS Number 304909-07-7
Molecular Formula

C23H27N5

Molecular Weight (MW) 373.49
SMILES CC1=C(C(=NN1C2=CC=CC=C2)C)C=NN3CCN(CC3)CC4=CC=CC=C4
InChIKey FOORCIAZMIWALX-JJIBRWJFSA-N

Ordering Information

Biological Activity

SANT-1 is a potent antagonist of the Smoothened (Smo) receptor, directly binding Smo with a Kd ofand inhibiting Smo agonist-induced activation with an IC50 of. By targeting Smo, SANT-1 suppresses Hedgehog signaling pathway activity, leading to down-regulation of Gli-mediated transcription and inhibition of proliferation in Hedgehog-dependent cells. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 75 mg/mL (200.8 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 18 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.