CAS No. 135239-65-5
Batch:
Purity:99.69
Chemical Structure
Check the
RP-64477
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | N-butyl-3-[(4-decoxybenzoyl)amino]-4-methylsulfanylbenzamide | |
|---|---|---|
| CAS Number | 135239-65-5 | |
| Molecular Formula |
C29H42N2O3S |
|
| Molecular Weight (MW) | 498.72 | |
| SMILES | CCCCCCCCCCOC1=CC=C(C=C1)C(=O)NC2=C(C=CC(=C2)C(=O)NCCCC)SC | |
| InChIKey | OZMWNRSILHTVFV-UHFFFAOYSA-N |
RP-64477 is a potent inhibitor of acyl-coenzyme A:cholesterol O-acyltransferase (ACAT), an enzyme central to intracellular cholesterol esterification. By inhibiting ACAT activity across intestinal, hepatic, and monocytic cell models, RP-64477 suppresses the esterification of free cholesterol into cholesteryl esters. This inhibition impairs cellular lipid storage, reduces apolipoprotein B secretion, and prevents foam cell formation, thereby altering cellular cholesterol homeostasis and lipid transport pathways. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(200.51 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.