Rotenone (Barbasco)

Name: Rotenone (Barbasco)
Brand: Selleck Chemicals
SKU: S2348
Rating: 5 (9 reviews)

For research use only.

Catalog No.S2348 Synonyms: Barbasco, Dactinol, Paraderil, Rotenon, Rotocide

9 publications

CAS No. 83-79-4

Rotenone (Barbasco, Dactinol, Paraderil, Rotenon, Rotocide) is a botanical insecticide that is an inhibitor of mitochondrial electron transport. Rotenone inhibits NADH/DB oxidoreductase and NADH oxidase with IC50 of 28.8 nM and 5.1 nM, respectively. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.

Selleck's Rotenone (Barbasco) has been cited by 9 publications

Cancer Lett, 2021, S0304-3835(21)00167-1

PubMed: 33872694 ( click the link to review the publication )

Curr Biol, 2021, S0960-9822(21)00626-6

PubMed: 34087103 ( click the link to review the publication )

Cancer Gene Ther, 2021, 10.1038/s41417-021-00348-y

PubMed: 34045663 ( click the link to review the publication )

G3 (Bethesda), 2020, 4;10(5):1585-1597

PubMed: 32265286 ( click the link to review the publication )

Oncol Rep, 2020, 44(1):103-114

PubMed: 32627037 ( click the link to review the publication )

Biomed Pharmacother, 2020, 129:110460

PubMed: 32768950 ( click the link to review the publication )

Nat Commun, 2019, 10(1):3856

PubMed: 31451693 ( click the link to review the publication )

Cancer Manag Res, 2019, 11:10145-10156

PubMed: 31819647 ( click the link to review the publication )

Mol Neurobiol, 2018, 55(5):4078-4089

PubMed: 28585189 ( click the link to review the publication )

Purity & Quality Control

Choose Selective NADPH-oxidase Inhibitors

Biological Activity

Description

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
HEK293	Mo\hSpVv[3Srb36gZZN{[Xl?			M{fYNWVn\mWldDDvckBE\GN{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[YZn\WO2IH;uJGNl[zJ8Q3TjNlVEKGmwdHXyZYN1cW:wIHPvcZBt\XinczDpckBxemW\|ZX7j[UBw\iClYX3weI91cGWlaX6gZpkhTVmIUDDhcoQwd3JiWV\QJHZmdnW\|IH\yZYdu\W62IHLhd4VlKHKncH;yeIVzKGenbnWgZZN{[Xl?	NIfHWI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNk[4NFE2QSd-MU[2PFAyPTl:L3G+
HEK293	MUHGeY5kfGmxbjDhd5NigQ>?			MUPF[oZm[3Rib36gR4RkOiCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHXm[oVkfCCxbjDD[IMzQkOmY{K1RUBqdnSncnHjeIlwdiClb33wcIV5\XNiaX6gdJJme2WwY3Wgc4Yh[2GvcITveIhm[2mwIHL5JGV[TlBiYX7kM49zKFmIUDDW[Y52eyCocnHncYVvfCCkYYPl[EBz\XCxcoTldkBo\W6nIHHzd4F6	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
HEK293	NV3IOIw3TnWwY4Tpc44h[XO\|YYm=			M2PWVmVn\mWldDDvckBxPTNiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDl[oZm[3Rib36gdFU{NXB3MzDpcpRmemGldHnvckBkd22ybHX4[ZMhcW5icILld4Vv[2Vib3[gZ4FueHSxdHjlZ4lvKGK7IFXZSnAh[W6mL3;yJHlHWCCYZX71d{BnemGpbXXueEBj[XOnZDDy[ZBwenSncjDn[Y5mKGG\|c3H5	MnjRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
HEK293	MofDSpVv[3Srb36gZZN{[Xl?			NV;5[ZR3UW6qaXLpeIlwdiCxZjDv[kBkNUq3bjDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iCycn;0[YlvKGmwdHXyZYN1cW:wIHnuJJBz\XOnbnPlJI9nKGOjbYD0c5Rp\WOrbjD3bZRpKFCrbkGgZpkhTVmIUDDiZZNm\CC{ZYDvdpRmeiCpZX7lJIF{e2G7	M3jCSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
HEK293	NY\vd49UTnWwY4Tpc44h[XO\|YYm=			Mn:3SYZn\WO2IH;uJINw\mmuaX6xJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO\|ZXSgZZMh\W[oZXP0JI9vKGOxZnnsbY4yQyCOaX3rNkBqdnSncnHjeIlwdiClb33wcIV5\XNiaX6gdJJme2WwY3Wgc4Yh[2GvcITveIhm[2mwIHL5JGV[TlBiYX7kM49zKFmIUDDW[Y52eyCocnHncYVvfCCkYYPl[EBz\XCxcoTldkBo\W6nIHHzd4F6	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
MG63	NHK2VYpHfW6ldHnvckBie3OjeR?=	NWrqe\|VlOSC3TR?=	M37FbFEhcHJ?	MlXGTY5kemWjc3WgbY4hdGGldHH0[UBxem:mdXP0bY9vKGmwIF3HOlMh[2WubIOgZZQhOSC3TTDh[pRmeiBzIHjyJIJ6KEWOSWPB	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzB6OES4PUc,OTdyOEi0PFk9N2F-
U937	Mln3RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MnvrO\|IhcHK\|	NVLsSY5uSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPVM4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3QuQCCjc4PhfUwhUUN3ME2wMlA4\|ryP	Mn31QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUiwOVQoRjF5MUW4NFU1RC:jPh?=
U937	NV7ncpRQSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MoH1OFghcHK\|	MWTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV7M{egZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFeVVD24JIF{e2G7LDDJR\|UxRTBwMt88US=>	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3OEC1OEc,OTdzNUiwOVQ9N2F-
U937	MnXuRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NWrKNm1VOjRiaILz	NH\Re5VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGW5N\|ch[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IGfTWE05KGG\|c3H5MEBKSzVyPUGz{txO	MoLvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUiwOVQoRjF5MUW4NFU1RC:jPh?=
HT1080	NUDtU4ljTnWwY4Tpc44h[XO\|YYm=			MWXJcohq[mm2aX;uJI9nKHC{b13NVFIheHKxZIXjeIlwdiCrbjDoeY1idiCKVEGwPFAh[2WubIOgZpkh\2WuYYTpckB7gW2xZ4LhdIh6KGGwYXz5d4l{	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3OEC1OEc,OTdzNUiwOVQ9N2F-
HT1080	M3PuV2Z2dmO2aX;uJIF{e2G7			Ml3LTY5pcWKrdHnvckBw\iCycn;NUXA6KHC{b3T1Z5Rqd25iaX6gbJVu[W5iSGSxNFgxKGOnbHzzJIJ6KGenbHH0bY4hgnmvb3fyZZBpgSCjbnHsfZNqew>?	NIL6TXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G1PFA2PCd-MUexOVgxPTR:L3G+
neural precursor cells	M3\y[2Z2dmO2aX;uJIF{e2G7			M3;KWWlvcGmkaYTpc44hd2ZibnX1do9{eGincnWgdJJwdGmoZYLheIlwdiCxZjDtc5V{\SCwZYXyZYwheHKnY4Xyd49zKGOnbHzzJIJ6KE2WVDDhd5NigQ>?	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzN{[zNUc,OTd2MUe2N\|E9N2F-
HepG2	MXvBcpRqfHWvb4KgZZN{[Xl?		MX2yOEBpenN?	NFG5W5VCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG\|c3H5MEBGTDVyPUG1{txO	NFKxRW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S4NlgzPCd-MUe0PFI5OjR:L3G+
A549	NGXtOWJCdnSrdIXtc5Ih[XO\|YYm=		M2XZeVI1KGi{cx?=	NW\FOFB6SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG\|c3H5MEBGTDVyPUK2{txO	Mo\TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2OEK4NlQoRjF5NEiyPFI1RC:jPh?=
N548	NX7OcIVNS2WubDDk[YF1cCC{ZYPjeYUh[XO\|YYm=			NWOzUXFpS2WubDDk[YF1cCC{ZYPjeYUhcW5icnH0JJN1emmjdHHsJI5mfXKxbjDOOVQ5KG23dHHueEBk\WyuczygSWM2OD1yLkZOwG0>	M{nMd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K2NFk5Lz5zN{eyOlA6QDxxYU6=
ASH neuronal cells	MUTD[YxtKGSnYYToJJJme2O3ZTDhd5NigQ>?			NFPlSIJE\WyuIHTlZZRpKHKnc3P1[UBw\iCDU1igcoV2em:wYXygZ4VtdHNiaX6gR4Fmdm:{aHHi[Il1cXNiZXzl[4FveyCKdX70bY5ofG:wJ4Og[Il{\WG\|ZTDtc4RmdCxiRVO1NF0z\|ryP	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzd{NkC5PEc,OTd5Mk[wPVg9N2F-
neuronal cells	NEXCNZVE\WyuIHTlZZRpKHKnc3P1[UBie3OjeR?=			MmrjR4VtdCCmZXH0bEBz\XOldXWgc4YhdmW3cn;uZYwh[2WubIOgbY4h\HKxc3;wbIlt[SCKdX70bY5ofG:wJ4Og[Il{\WG\|ZTDtc4RmdCxiRVO1NF0yOM7:TR?=	NXiyOoRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NlYxQThpPkG3O\|I3ODl6PD;hQi=>
N548	NGjKdZpVd3irY3n0fUBie3OjeR?=			MVXUc5hq[2m2eTDpckBz[XRic4TybYF1[WxibnX1do9v[WxiTkW0PEBufXSjboSgZ4VtdHNuIGTDOVA:PDEQvF2=	MlXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd5Mk[wPVgoRjF5N{K2NFk5RC:jPh?=
N548	M4jucmNmdGxiZHXheIghemW\|Y4XlJIF{e2G7			MVXTeZBxemW\|c3nvckBw\iClZXzsJIRm[XSqIHnuJJJifCC\|dILpZZRidCCwZYXyc45idCCQNUS4JI12fGGwdDDj[YxteyCjc4Pld5Nm\CCjczDzeZBxemW\|c3nvckBw\iClYYPwZZNmKDdiYXP0bZZifGmxbjDifUBY\XO2ZYLuJIJtd3S2aX7n	NUXaXYdsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NlYxQThpPkG3O\|I3ODl6PD;hQi=>
N548	MUjBZ5RqfmG2aX;uJI9nKEWUSzDpci=>	MVWxNEB2VQ>?		NIr1ZZlC[3SrdnH0bY9vKG:oIFXST{BqdiC{YYSgd5RzcWG2YXygcoV2em:wYXygUlU1QCCvdYThcpQh[2WubIOgZZQhOTBidV2gZpkhX2W\|dHXyckBjdG:2dHnu[y=>	NVz0UoRPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NlYxQThpPkG3O\|I3ODl6PD;hQi=>
N548	NYi1RmNkSWO2aY\heIlwdiCxZjDBT3QhcW5?	M17jflExKHWP		NI\RTI5C[3SrdnH0bY9vKG:oIFHLWEBqdiC{YYSgd5RzcWG2YXygcoV2em:wYXygUlU1QCCvdYThcpQh[2WubIOgZZQhOTBidV2gZpkhX2W\|dHXyckBjdG:2dHnu[y=>	MkDzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd5Mk[wPVgoRjF5N{K2NFk5RC:jPh?=
N548	MoLoRYN1cX[jdHnvckBw\iCHUlugbY4>	NF\USVI2OCC3TR?=		MYnBZ5RqfmG2aX;uJI9nKEWUSzDpckBz[XRic4TybYF1[WxibnX1do9v[WxiTkW0PEBufXSjboSgZ4VtdHNiYYSgOVAhfU1iYomgW4V{fGW{bjDicI91fGmwZx?=	NIrzO3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{eyOlA6QCd-MUe3NlYxQTh:L3G+
N548	NH;NVXBC[3SrdnH0bY9vKG:oIFHLWEBqdg>?	M3XESVUxKHWP		MknRRYN1cX[jdHnvckBw\iCDS2SgbY4hemG2IIP0dolifGGuIH7leZJwdmGuIF61OFghdXW2YX70JINmdGy\|IHH0JFUxKHWPIHL5JHdme3Sncn6gZoxwfHSrbne=	NXLWc3p3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NlYxQThpPkG3O\|I3ODl6PD;hQi=>
N548	MnH6RYN1cX[jdHnvckBw\iCDS2SgbY4>	Moe0NVAhfU1?	MmnSOkBpenN?	NFXIT2xC[3SrdnH0bY9vKG:oIFHLWEBqdiCGTlGt[IVncWOrZX70JJJifCC\|dILpZZRidCCwZYXyc45idCCQNUS4JI12fGGwdDDj[YxteyCjdDCxNEB2VSCjZoTldkA3KGi{czDifUBY\XO2ZYLuJIJtd3S2aX7n	NGTpZos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{eyOlA6QCd-MUe3NlYxQTh:L3G+
N548	MkXYR4VtdCCmZXH0bEBz\XOldXWgZZN{[Xl?			MUnD[YxtKGSnYYToJJJme2O3ZTDpckBz[XRic4TybYF1[WxibnX1do9v[WxiTkW0PEBufXSjboSgZ4VtdHNiYomgZ4Ft[2WrbjDhZ4V1d3i7bXX0bJltKGW\|dHXyJIF{e2G7	NG\oV2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{eyOlA6QCd-MUe3NlYxQTh:L3G+
N548	NITqc2hE\WyuIHTlZZRpKHKnc3P1[UBie3OjeR?=	NVK3dVR{OTBidV2=	NI\CSGY4KGSjeYO=	NF7uS4NE\WyuIHTlZZRpKHKnc3P1[UBqdiC{YYSgd5RzcWG2YXygcoV2em:wYXygUlU1QCCvdYThcpQh[2WubIOgZY5idHm\|ZXSg[ZZmenliZHH5JIZweiB5IHThfZMh[XRiMUCgeW0h[nlidIL5dIFvKGKudXWg[JlmNWW6Y3z1d4lwdiCjc4PhfS=>	NH7xT3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{eyOlA6QCd-MUe3NlYxQTh:L3G+
N548	NYXFN2tlS2WubDDk[YF1cCC{ZYPjeYUh[XO\|YYm=			Mn6xV5VxeHKnc4Ppc44hd2ZiY3XscEBl\WG2aDDpckBz[XRic4TybYF1[WxibnX1do9v[WxiTkW0PEBufXSjboSgZ4VtdHNiYYPz[ZN{\WRiYYOgd5VxeHKnc4Ppc44hd2ZiY3HzdIF{\SB\|IHHjeIl3[XSrb36gZpkhX2W\|dHXyckBjdG:2dHnu[y=>	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzd{NkC5PEc,OTd5Mk[wPVg9N2F-
N548	NXrOToIxS2WubDDk[YF1cCC{ZYPjeYUh[XO\|YYm=	MoniNVAhfU1?		NUKyOGN3S2WubDDk[YF1cCC{ZYPjeYUhcW5iRF7BMYRm\mmlaXXueEBz[XRic4TybYF1[WxibnX1do9v[WxiTkW0PEBufXSjboSgZ4VtdHNiYYSgNVAhfU1?	M{\IOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K2NFk5Lz5zN{eyOlA6QDxxYU6=
U373 MG	NFfmNI5CdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MoDzO\|IhcHK\|	MXjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV\|N{OgUWch[2WubIOgZYZ1\XJiN{KgbJJ{	NEPOW5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEWxN\|k4PCd-MUi1NVM6PzR:L3G+
T47D	MoDaSpVv[3Srb36gZZN{[Xl?	M3zSPVAvODBzIITvJFAvOSC3TR?=		NFrhXmJKdmirYnn0bY9vKG:oIHPlcIx2dGG{IILld5BqemG2aX;uJIlvKGi3bXHuJHQ1P0RiY3XscJMh[XO\|ZYPz[YQh[XNicnH0[UBw\iCxeInn[Y4h[2:wc4XtdJRqd25iYYSgNE4xODFidH:gNE4yKHWP	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh5NUGxOEc,OjF6N{WxNVQ9N2F-
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TC32	MX\xTHRUKGG\|c3H5			M1PjUZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM>	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
MG 63 (6-TG R)	MlLndWhVWyCjc4PhfS=>			Ml3IdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUWchPjNiKE[tWGchWiliY3XscJM>	M{DET\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
fibroblast cells	M4PNV5FJXFNiYYPzZZk>			Mln1dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgZ49vfHKxbDDIbEB4cWymIIT5dIUh\mmkcn;icIF{fCClZXzsdy=>	M{XmUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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HepG2	MYXDfZRwfG:6aXPpeJkh[XO\|YYm=		NXf0NJBEOjRiaILz	M3jVe2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU42Os7:TR?=	NYPXU5RJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C2NVM{OjdpPkOwOlE{OzJ5PD;hQi=>
DLD1	M3\UVWZ2dmO2aX;uJIF{e2G7			NHTwUGVKdmirYnn0bY9vKG:oIH3peI9kcG:wZILpZYwhd3irZHH0bZZmKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBFVERzIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBwgHmpZX6gZ49ve3WvcITpc44hemG2ZTDifUB{\WGqb4Lz[UBZTmV7NjDhcoFtgXOncjDiZZNm\CCjc4PhfS=>	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTd5NE[3Nkc,OzF5N{S2O\|I9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	sestrin2 ; PubMed: 26031332 Mol Cell Biol Mes 23.5 cells were treated with DMSO or rotenone at the indicated concentrations for 12 h. After rotenone treatment, cells were harvested and immunoblotted for sestrin2 protein levels. p-AMPK / AMPK ; PubMed: 26031332 Mol Cell Biol Cells were treated with rotenone and immunoblotted for phosphorylated AMPK (Thr172) and total AMPK. p-mTOR / mTOR / p-S6K1 / S6K1 / p-4EBP1 / 4EBP1 ; PubMed: 25304210 Toxicol Sci Neuronal cells were treated with (A) rotenone (0–1 μM) for 24 h followed by Western blotting using the indicated antibodies. The blots were probed for β-tubulin as a loading control. p-CaMKII / CaMKII ; PubMed: 26859572 Oncotarget Rotenone resulted in phospho-CaMKII increase in a concentration-dependent manner in the cells.	26031332 25304210 26859572
Immunofluorescence	p65 ; PubMed: 23977201 PLoS One BV2 cells were treated with rotenone for 6 hours or LPS for 1 hour. The cells were fixed and labeled with DAPI (blue) or anti-p65 antibodies (red). The scale bar represents 10 µm. activated caspase-1 ; PubMed: 23977201 PLoS One BV2 cells were treated with 1 µM rotenone or 1 µg/mL LPS for 24 hours and then stained with green fluorescent probes for activated caspase-1 for 1 hour. The scale bar represents 10 µm.	23977201
Growth inhibition assay	Cell viability; PubMed: 27045591 PLoS One PC12 cells were treated with 0~100 μmol/L (μM) rotenone for 24 hours. Cell viability was measured as percentage relative to vehicle (P < 0.05; *P < 0.01).	27045591

Protocol

References

Solubility (25°C)

In vitro	DMSO	55 mg/mL (139.44 mM)
	Ethanol	2 mg/mL (5.07 mM)
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Rotenone (Barbasco) SDF

Molecular Weight	394.42
Formula	C₂₃H₂₂O₆
CAS No.	83-79-4
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	Barbasco, Dactinol, Paraderil, Rotenon, Rotocide
Smiles	CC(=C)C1CC2=C(O1)C=CC3=C2OC4COC5=CC(=C(C=C5C4C3=O)OC)OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Rotenone (Barbasco)