Rotenone (Barbasco)

For research use only.

Catalog No.S2348 Synonyms: Barbasco, Dactinol, Paraderil, Rotenon, Rotocide

9 publications

Rotenone (Barbasco) Chemical Structure

CAS No. 83-79-4

Rotenone (Barbasco, Dactinol, Paraderil, Rotenon, Rotocide) is a botanical insecticide that is an inhibitor of mitochondrial electron transport. Rotenone inhibits NADH/DB oxidoreductase and NADH oxidase with IC50 of 28.8 nM and 5.1 nM, respectively. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.

Selleck's Rotenone (Barbasco) has been cited by 9 publications

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Biological Activity

Description Rotenone (Barbasco, Dactinol, Paraderil, Rotenon, Rotocide) is a botanical insecticide that is an inhibitor of mitochondrial electron transport. Rotenone inhibits NADH/DB oxidoreductase and NADH oxidase with IC50 of 28.8 nM and 5.1 nM, respectively. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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HEK293 MUHGeY5kfGmxbjDhd5NigQ>? MUPF[oZm[3Rib36gR4RkOiCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHXm[oVkfCCxbjDD[IMzQkOmY{K1RUBqdnSncnHjeIlwdiClb33wcIV5\XNiaX6gdJJme2WwY3Wgc4Yh[2GvcITveIhm[2mwIHL5JGV[TlBiYX7kM49zKFmIUDDW[Y52eyCocnHncYVvfCCkYYPl[EBz\XCxcoTldkBo\W6nIHHzd4F6 MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
HEK293 NV3IOIw3TnWwY4Tpc44h[XO|YYm= M2PWVmVn\mWldDDvckBxPTNiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDl[oZm[3Rib36gdFU{NXB3MzDpcpRmemGldHnvckBkd22ybHX4[ZMhcW5icILld4Vv[2Vib3[gZ4FueHSxdHjlZ4lvKGK7IFXZSnAh[W6mL3;yJHlHWCCYZX71d{BnemGpbXXueEBj[XOnZDDy[ZBwenSncjDn[Y5mKGG|c3H5 MnjRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
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HEK293 NY\vd49UTnWwY4Tpc44h[XO|YYm= Mn:3SYZn\WO2IH;uJINw\mmuaX6xJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMh\W[oZXP0JI9vKGOxZnnsbY4yQyCOaX3rNkBqdnSncnHjeIlwdiClb33wcIV5\XNiaX6gdJJme2WwY3Wgc4Yh[2GvcITveIhm[2mwIHL5JGV[TlBiYX7kM49zKFmIUDDW[Y52eyCocnHncYVvfCCkYYPl[EBz\XCxcoTldkBo\W6nIHHzd4F6 MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
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fibroblast cells M4PNV5FJXFNiYYPzZZk> Mln1dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgZ49vfHKxbDDIbEB4cWymIIT5dIUh\mmkcn;icIF{fCClZXzsdy=> M{XmUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 NEjhdFdyUFSVIHHzd4F6 NIPzU4xyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBQUFNvNUCgZ4VtdHN? NX\JPIVyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
HepG2 MYXDfZRwfG:6aXPpeJkh[XO|YYm= NXf0NJBEOjRiaILz M3jVe2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU42Os7:TR?= NYPXU5RJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C2NVM{OjdpPkOwOlE{OzJ5PD;hQi=>
DLD1 M3\UVWZ2dmO2aX;uJIF{e2G7 NHTwUGVKdmirYnn0bY9vKG:oIH3peI9kcG:wZILpZYwhd3irZHH0bZZmKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBFVERzIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBwgHmpZX6gZ49ve3WvcITpc44hemG2ZTDifUB{\WGqb4Lz[UBZTmV7NjDhcoFtgXOncjDiZZNm\CCjc4PhfS=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTd5NE[3Nkc,OzF5N{S2O|I9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
sestrin2 ; 

PubMed: 26031332     


Mes 23.5 cells were treated with DMSO or rotenone at the indicated concentrations for 12 h. After rotenone treatment, cells were harvested and immunoblotted for sestrin2 protein levels.

p-AMPK / AMPK ; 

PubMed: 26031332     


Cells were treated with rotenone and immunoblotted for phosphorylated AMPK (Thr172) and total AMPK. 

p-mTOR / mTOR / p-S6K1 / S6K1 / p-4EBP1 / 4EBP1 ; 

PubMed: 25304210     


Neuronal cells were treated with (A) rotenone (0–1 μM) for 24 h followed by Western blotting using the indicated antibodies. The blots were probed for β-tubulin as a loading control. 

p-CaMKII / CaMKII ; 

PubMed: 26859572     


Rotenone resulted in phospho-CaMKII increase in a concentration-dependent manner in the cells.

26031332 25304210 26859572
Immunofluorescence
p65 ; 

PubMed: 23977201     


BV2 cells were treated with rotenone for 6 hours or LPS for 1 hour. The cells were fixed and labeled with DAPI (blue) or anti-p65 antibodies (red). The scale bar represents 10 µm.

activated caspase-1 ; 

PubMed: 23977201     


BV2 cells were treated with 1 µM rotenone or 1 µg/mL LPS for 24 hours and then stained with green fluorescent probes for activated caspase-1 for 1 hour. The scale bar represents 10 µm.

23977201
Growth inhibition assay
Cell viability; 

PubMed: 27045591     


PC12 cells were treated with 0~100 μmol/L (μM) rotenone for 24 hours. Cell viability was measured as percentage relative to vehicle (*P < 0.05; **P < 0.01).

27045591

Protocol

References

Solubility (25°C)

In vitro DMSO 55 mg/mL (139.44 mM)
Ethanol 2 mg/mL (5.07 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 394.42
Formula

C23H22O6

CAS No. 83-79-4
Storage powder
in solvent
Synonyms Barbasco, Dactinol, Paraderil, Rotenon, Rotocide
Smiles CC(=C)C1CC2=C(O1)C=CC3=C2OC4COC5=CC(=C(C=C5C4C3=O)OC)OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Step 2: Enter the in vivo formulation ()
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID