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Rotenone (Barbasco)

Name: Rotenone (Barbasco)
Brand: Selleck Chemicals
SKU: S2348
Rating: 5 (14 reviews)

Catalog No.S2348 Synonyms: Barbasco, Dactinol, Paraderil, Rotenon, Rotocide

For research use only.

Rotenone (Barbasco, Dactinol, Paraderil, Rotenon, Rotocide) is a botanical insecticide that is an inhibitor of mitochondrial electron transport. Rotenone inhibits NADH/DB oxidoreductase and NADH oxidase with IC50 of 28.8 nM and 5.1 nM, respectively. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.

CAS No. 83-79-4

Selleck's Rotenone (Barbasco) has been cited by 14 publications

Purity & Quality Control

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Biological Activity

Description

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

HEK293	NEDNb2FHfW6ldHnvckBie3OjeR?=			NE\HdlJG\m[nY4Sgc44hS2SlMjDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHHzd4V{e2WmIHHzJIVn\mWldDDvckBE\GN{OlPkZ\|I2SyCrboTldoFkfGmxbjDjc41xdGW6ZYOgbY4heHKnc3XuZ4Uhd2ZiY3HtdJRwfGinY3nuJIJ6KEW\RmCgZY5lN2:{IGnGVEBX\W63czDmdoFodWWwdDDiZZNm\CC{ZYDvdpRmeiCpZX7lJIF{e2G7	Mo\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
HEK293	MWPGeY5kfGmxbjDhd5NigQ>?			NHHTdJNG\m[nY4Sgc44hS2SlMjDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHHzd4V{e2WmIHHzJIVn\mWldDDvckBE\GN{OlPkZ\|I2SSCrboTldoFkfGmxbjDjc41xdGW6ZYOgbY4heHKnc3XuZ4Uhd2ZiY3HtdJRwfGinY3nuJIJ6KEW\RmCgZY5lN2:{IGnGVEBX\W63czDmdoFodWWwdDDiZZNm\CC{ZYDvdpRmeiCpZX7lJIF{e2G7	M171N\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
HEK293	MlzhSpVv[3Srb36gZZN{[Xl?			MWnF[oZm[3Rib36gdFU{KGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO\|ZYPz[YQh[XNiZX\m[YN1KG:wIIC1N{1xPTNiaX70[ZJi[3Srb36gZ49ueGyneHXzJIlvKHC{ZYPlcoNmKG:oIHPhcZB1d3SqZXPpckBjgSCHWV\QJIFv\C:xcjDZSnAhXmWwdYOg[pJi\22nboSgZoF{\WRicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
HEK293	MWXGeY5kfGmxbjDhd5NigQ>?			NHG5VW1KdmirYnn0bY9vKG:oIH;mJIMuUnWwIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKHC{b4TlbY4hcW62ZYLhZ5Rqd25iaX6gdJJme2WwY3Wgc4Yh[2GvcITveIhm[2mwIIfpeIghWGmwMTDifUBGYU[SIHLhd4VlKHKncH;yeIVzKGenbnWgZZN{[Xl?	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
HEK293	NYPQdW9ETnWwY4Tpc44h[XO\|YYm=			NGfjfZBG\m[nY4Sgc44h[2:oaXzpclEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bie3Onc4Pl[EBieyCnZn\lZ5Qhd25iY3;mbYxqdjF9IFzpcYszKGmwdHXyZYN1cW:wIHPvcZBt\XinczDpckBxemW\|ZX7j[UBw\iClYX3weI91cGWlaX6gZpkhTVmIUDDhcoQwd3JiWV\QJHZmdnW\|IH\yZYdu\W62IHLhd4VlKHKncH;yeIVzKGenbnWgZZN{[Xl?	NIW4bIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNk[4NFE2QSd-MU[2PFAyPTl:L3G+
MG63	Mn\2SpVv[3Srb36gZZN{[Xl?	MofKNUB2VQ>?	NFvncVUyKGi{	NFW3OopKdmO{ZXHz[UBqdiCuYXP0ZZRmKHC{b3T1Z5Rqd25iaX6gUWc3OyClZXzsd{BifCBzIIXNJIFnfGW{IEGgbJIh[nliRVzJV2E>	M4ruS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEi4OFg6Lz5zN{C4PFQ5QTxxYU6=
U937	MVrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NVPGO3E1PzJiaILz	M3\S[2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVUmzO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliV2PUMVgh[XO\|YYmsJGlEPTB;MD6wO:69VQ>?	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3OEC1OEc,OTdzNUiwOVQ9N2F-
U937	MV3BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MY[0PEBpenN?	M1\GNmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVUmzO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliV2PUMVgh[XO\|YYmsJGlEPTB;MD6y{txO	M3;1UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUW4NFU1Lz5zN{G1PFA2PDxxYU6=
U937	NFr5N21CdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MX2yOEBpenN?	MXLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV7M{egZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFeVVD24JIF{e2G7LDDJR\|UxRTF\|zszN	M3fIVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUW4NFU1Lz5zN{G1PFA2PDxxYU6=
HT1080	MVnGeY5kfGmxbjDhd5NigQ>?			M1HqWWlvcGmkaYTpc44hd2ZicILvUW1ROiCycn;keYN1cW:wIHnuJIh2dWGwIFjUNVA5OCClZXzsd{BjgSCpZXzheIlvKHq7bX;ndoFxcHliYX7hcJl{cXN?	M1\xfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUW4NFU1Lz5zN{G1PFA2PDxxYU6=
HT1080	NXzaOIN1TnWwY4Tpc44h[XO\|YYm=			M2Hld2lvcGmkaYTpc44hd2ZicILvUW1RQSCycn;keYN1cW:wIHnuJIh2dWGwIFjUNVA5OCClZXzsd{BjgSCpZXzheIlvKHq7bX;ndoFxcHliYX7hcJl{cXN?	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3OEC1OEc,OTdzNUiwOVQ9N2F-
neural precursor cells	Mn7USpVv[3Srb36gZZN{[Xl?			MY\Jcohq[mm2aX;uJI9nKG6ndYLvd5Bp\XKnIIDyc4xq\mW{YYTpc44hd2ZibX;1d4UhdmW3cnHsJJBz\WO3coPvdkBk\WyuczDifUBOXFRiYYPzZZk>	NVjrW2Z7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVc3OzFpPkG3OFE4PjNzPD;hQi=>
HepG2	MYnBcpRqfHWvb4KgZZN{[Xl?		MnrCNlQhcHK\|	M4riNmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO\|YYmsJGVFPTB;MUZOwG0>	NY[wU2NiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0PFI5OjRpPkG3OFgzQDJ2PD;hQi=>
A549	MnniRY51cXS3bX;yJIF{e2G7		M1XoVVI1KGi{cx?=	M1TDdWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygSWQ2OD1{Nt88US=>	NIfCWY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S4NlgzPCd-MUe0PFI5OjR:L3G+
N548	NWHCcmc6S2WubDDk[YF1cCC{ZYPjeYUh[XO\|YYm=			NGfwNVNE\WyuIHTlZZRpKHKnc3P1[UBqdiC{YYSgd5RzcWG2YXygcoV2em:wIF61OFghdXW2YX70JINmdGy\|LDDFR\|UxRTBwNd88US=>	M2ftS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K2NFk5Lz5zN{eyOlA6QDxxYU6=
ASH neuronal cells	NYW0[FdES2WubDDk[YF1cCC{ZYPjeYUh[XO\|YYm=			NFvWbHpE\WyuIHTlZZRpKHKnc3P1[UBw\iCDU1igcoV2em:wYXygZ4VtdHNiaX6gR4Fmdm:{aHHi[Il1cXNiZXzl[4FveyCKdX70bY5ofG:wJ4Og[Il{\WG\|ZTDtc4RmdCxiRVO1NF0z\|ryP	M1PaWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K2NFk5Lz5zN{eyOlA6QDxxYU6=
neuronal cells	M3vucGNmdGxiZHXheIghemW\|Y4XlJIF{e2G7			M1vlOGNmdGxiZHXheIghemW\|Y4XlJI9nKG6ndYLvcoFtKGOnbHzzJIlvKGS{b4PvdIhqdGFiSIXueIlv\3SxbjfzJIRqe2Wjc3WgcY9l\WxuIFXDOVA:OTEQvF2=	NUHMWoYzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NlYxQThpPkG3O\|I3ODl6PD;hQi=>
N548	MoTNWI95cWOrdImgZZN{[Xl?			MXTUc5hq[2m2eTDpckBz[XRic4TybYF1[WxibnX1do9v[WxiTkW0PEBufXSjboSgZ4VtdHNuIGTDOVA:PDEQvF2=	M4TT[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K2NFk5Lz5zN{eyOlA6QDxxYU6=
N548	NUTCSmNKS2WubDDk[YF1cCC{ZYPjeYUh[XO\|YYm=			NVGzdWNsW3WycILld5Nqd25ib3[gZ4VtdCCmZXH0bEBqdiC{YYSgd5RzcWG2YXygcoV2em:wYXygUlU1QCCvdYThcpQh[2WubIOgZZN{\XO\|ZXSgZZMhe3WycILld5Nqd25ib3[gZ4F{eGG\|ZTC3JIFkfGm4YYTpc44h[nliV3XzeIVzdiCkbH;0eIlv\w>?	NF7aZ3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{eyOlA6QCd-MUe3NlYxQTh:L3G+
N548	Ml22RYN1cX[jdHnvckBw\iCHUlugbY4>	MWCxNEB2VQ>?		MXPBZ5RqfmG2aX;uJI9nKEWUSzDpckBz[XRic4TybYF1[WxibnX1do9v[WxiTkW0PEBufXSjboSgZ4VtdHNiYYSgNVAhfU1iYomgW4V{fGW{bjDicI91fGmwZx?=	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzd{NkC5PEc,OTd5Mk[wPVg9N2F-
N548	NWrGWHoySWO2aY\heIlwdiCxZjDBT3QhcW5?	M3zib\|ExKHWP		NGSxNVNC[3SrdnH0bY9vKG:oIFHLWEBqdiC{YYSgd5RzcWG2YXygcoV2em:wYXygUlU1QCCvdYThcpQh[2WubIOgZZQhOTBidV2gZpkhX2W\|dHXyckBjdG:2dHnu[y=>	M3;CeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K2NFk5Lz5zN{eyOlA6QDxxYU6=
N548	NVrjU5V7SWO2aY\heIlwdiCxZjDFVmshcW5?	M4TPOFUxKHWP		NGLIfZJC[3SrdnH0bY9vKG:oIFXST{BqdiC{YYSgd5RzcWG2YXygcoV2em:wYXygUlU1QCCvdYThcpQh[2WubIOgZZQhPTBidV2gZpkhX2W\|dHXyckBjdG:2dHnu[y=>	NIPKWJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{eyOlA6QCd-MUe3NlYxQTh:L3G+
N548	M3;McmFkfGm4YYTpc44hd2ZiQVvUJIlv	NFXTU3M2OCC3TR?=		NFLOU5NC[3SrdnH0bY9vKG:oIFHLWEBqdiC{YYSgd5RzcWG2YXygcoV2em:wYXygUlU1QCCvdYThcpQh[2WubIOgZZQhPTBidV2gZpkhX2W\|dHXyckBjdG:2dHnu[y=>	MkexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd5Mk[wPVgoRjF5N{K2NFk5RC:jPh?=
N548	NX73cmM5SWO2aY\heIlwdiCxZjDBT3QhcW5?	NFLafpYyOCC3TR?=	MkX4OkBpenN?	NELSdmJC[3SrdnH0bY9vKG:oIFHLWEBqdiCGTlGt[IVncWOrZX70JJJifCC\|dILpZZRidCCwZYXyc45idCCQNUS4JI12fGGwdDDj[YxteyCjdDCxNEB2VSCjZoTldkA3KGi{czDifUBY\XO2ZYLuJIJtd3S2aX7n	Mn;YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd5Mk[wPVgoRjF5N{K2NFk5RC:jPh?=
N548	MmrSR4VtdCCmZXH0bEBz\XOldXWgZZN{[Xl?			NVTMPZpvS2WubDDk[YF1cCC{ZYPjeYUhcW5icnH0JJN1emmjdHHsJI5mfXKxbnHsJG42PDhibYX0ZY51KGOnbHzzJIJ6KGOjbHPlbY4h[WOndH;4fY1mfGi7bDDld5RmeiCjc4PhfS=>	NFTOWZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{eyOlA6QCd-MUe3NlYxQTh:L3G+
N548	NEP2ZVlE\WyuIHTlZZRpKHKnc3P1[UBie3OjeR?=	M{PNWVExKHWP	NEDGVo04KGSjeYO=	NWnKfnNES2WubDDk[YF1cCC{ZYPjeYUhcW5icnH0JJN1emmjdHHsJI5mfXKxbnHsJG42PDhibYX0ZY51KGOnbHzzJIFv[Wy7c3XkJIV3\XK7IHThfUBnd3JiNzDkZZl{KGG2IEGwJJVOKGK7IITyfZBidiCkbIXlJIR6\S2neHPseZNqd25iYYPzZZk>	M3W2ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K2NFk5Lz5zN{eyOlA6QDxxYU6=
N548	NYDo[m9KS2WubDDk[YF1cCC{ZYPjeYUh[XO\|YYm=			NGO5cpZUfXCycnXzd4lwdiCxZjDj[YxtKGSnYYToJIlvKHKjdDDzeJJq[XSjbDDu[ZVzd26jbDDOOVQ5KG23dHHueEBk\WyuczDhd5Nme3OnZDDhd{B{fXCycnXzd4lwdiCxZjDjZZNx[XOnIEOgZYN1cX[jdHnvckBjgSCZZYP0[ZJvKGKub4T0bY5o	NHi2eHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{eyOlA6QCd-MUe3NlYxQTh:L3G+
N548	MkDFR4VtdCCmZXH0bEBz\XOldXWgZZN{[Xl?	MXixNEB2VQ>?		NHm0T4tE\WyuIHTlZZRpKHKnc3P1[UBqdiCGTlGt[IVncWOrZX70JJJifCC\|dILpZZRidCCwZYXyc45idCCQNUS4JI12fGGwdDDj[YxteyCjdDCxNEB2VQ>?	M1\aNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K2NFk5Lz5zN{eyOlA6QDxxYU6=
U373 MG	M2CzUWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NWHCOIdYPzJiaILz	M4TsbmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVUO3N{BOTyClZXzsd{Bi\nSncjC3NkBpenN?	NVn3U\|J6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NVM6PzRpPkG4OVE{QTd2PD;hQi=>
T47D	Mnu4SpVv[3Srb36gZZN{[Xl?	M{DHd\|AvODBzIITvJFAvOSC3TR?=		NWnFSIE4UW6qaXLpeIlwdiCxZjDj[YxtfWyjcjDy[ZNxcXKjdHnvckBqdiCqdX3hckBVPDeGIHPlcIx{KGG\|c3Xzd4VlKGG\|IILheIUhd2Zib4j5[4VvKGOxboP1cZB1cW:wIHH0JFAvODBzIITvJFAvOSC3TR?=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh5NUGxOEc,OjF6N{WxNVQ9N2F-
T47D	MkX4SpVv[3Srb36gZZN{[Xl?		M1XCT\|Q5KGi{cx?=	M3nhTXJm\HWldHnvckBqdiCDVGCgcIV3\WxiaX6gbJVu[W5iVES3SEBk\WyuczDh[pRmeiB2ODDodpMh[nliSGDMR{1WXiCjbnHsfZNqew>?	MkjwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{WxNVQoRjJzOEe1NVE1RC:jPh?=
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BT-37	NGrnUlByUFSVIHHzd4F6			NHjqTXNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1\|NzDj[Yxtew>?	M4rqNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32	NFfMPY1yUFSVIHHzd4F6			NXH6VlBjeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN\|MjDj[Yxtew>?	NHvDbYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
MG 63 (6-TG R)	NWPoVlhjeUiWUzDhd5NigQ>?			NX3re2hteUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN?	NXXM[XZpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
fibroblast cells	NWDKeVVQeUiWUzDhd5NigQ>?			MV;xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDjc451em:uIFjoJJdqdGRidInw[UBncWK{b3LsZZN1KGOnbHzz	NUPXNotiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
OHS-50	NIP1RnVyUFSVIHHzd4F6			MknudWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgU2hUNTVyIHPlcIx{	NGTjOGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
HepG2	MmmwR5l1d3SxeHnjbZR6KGG\|c3H5		MWOyOEBpenN?	NIfQN5JEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvPTMQvF2=	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODZzM{OyO{c,OzB4MUOzNlc9N2F-
DLD1	NHvmfphHfW6ldHnvckBie3OjeR?=			MXvJcohq[mm2aX;uJI9nKG2rdH;jbI9v\HKrYXygc5hq\GG2aY\lJJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDEUGQyKGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDvfJlo\W5iY3;ud5VueHSrb36gdoF1\SCkeTDz[YFpd3K\|ZTDYSoU6PiCjbnHsfZNmeiCkYYPl[EBie3OjeR?=	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTd5NE[3Nkc,OzF5N{S2O\|I9N2F-

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	sestrin2 ; p-AMPK / AMPK ; p-mTOR / mTOR / p-S6K1 / S6K1 / p-4EBP1 / 4EBP1 ; p-CaMKII / CaMKII	26031332 25304210 26859572
Immunofluorescence	p65 ; activated caspase-1	23977201
Growth inhibition assay	Cell viability	27045591

Protocol (from reference)

Solubility (25°C)

In vitro	DMSO	55 mg/mL (139.44 mM)
	Ethanol	2 mg/mL (5.07 mM)
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	394.42
Formula	C₂₃H₂₂O₆
CAS No.	83-79-4
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC(=C)C1CC2=C(O1)C=CC3=C2OC4COC5=CC(=C(C=C5C4C3=O)OC)OC

Download Rotenone (Barbasco) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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