CAS No. 1062243-51-9

Batch: Purity:99.87
Ro3280 Chemical Structure
Chemical Structure
Check the Ro3280 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-[(9-cyclopentyl-7,7-difluoro-5-methyl-6-oxo-8H-pyrimido[4,5-b][1,4]diazepin-2-yl)amino]-3-methoxy-N-(1-methylpiperidin-4-yl)benzamide
CAS Number 1062243-51-9
Molecular Formula

C27H35F2N7O3

Molecular Weight (MW) 543.61
SMILES CN1CCC(CC1)NC(=O)C2=CC(=C(C=C2)NC3=NC=C4C(=N3)N(CC(C(=O)N4C)(F)F)C5CCCC5)OC
InChIKey DJNZZLZKAXGMMC-UHFFFAOYSA-N

Ordering Information

Biological Activity

Ro3280 is a potent and selective inhibitor of Polo-like kinase 1 (PLK1) with an IC50 of 3 nM. Targeted inhibition of PLK1 disrupts mitotic spindle assembly and chromosome alignment during mitosis, leading to cell cycle arrest at the G2/M phase. Downstream, this persistent mitotic blockade impairs tumor cell proliferation and promotes programmed cell death through activation of apoptotic signaling pathways.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (183.95 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 100 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Aliquotting Service

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Note: Technical data last updated: Sep 1, 2026.