CAS No. 81-88-9
Batch:
Purity:98.91
Chemical Structure
Check the
Rhodamine B
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | [9-(2-carboxyphenyl)-6-(diethylamino)xanthen-3-ylidene]-diethylazanium chloride | |
|---|---|---|
| CAS Number | 81-88-9 | |
| Molecular Formula |
C28H31ClN2O3 |
|
| Molecular Weight (MW) | 479.01 | |
| SMILES | CCN(CC)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](CC)CC)C=C3O2)C4=CC=CC=C4C(=O)O.[Cl-] | |
| InChIKey | PYWVYCXTNDRMGF-UHFFFAOYSA-N |
Rhodamine B is a xanthene fluorophore and fluorescent dye used as a biological stain and tracer. It serves as an imaging probe in fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy, and immunoassays to evaluate organelle localization and cellular uptake. Its high fluorescence yield and membrane permeability enable the tracking of fluid flow, membrane dynamics, and transport kinetics across biological systems without requiring specific target inhibition.
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Concentration
Volume
Mass
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|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
96 mg/mL
(200.41 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.