CAS No. 54-47-7

Batch: Purity:99.84
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Pyridoxal phosphate Chemical Structure
Chemical Structure
Check the Pyridoxal phosphate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (4-formyl-5-hydroxy-6-methyl-3-pyridinyl)methyl dihydrogen phosphate
CAS Number 54-47-7
Molecular Formula

C8H10NO6P
Molecular Weight (MW) 247.14
SMILES CC1=NC=C(C(=C1O)C=O)COP(=O)(O)O
InChIKey NGVDGCNFYWLIFO-UHFFFAOYSA-N

Ordering Information

Biological Activity

Pyridoxal phosphate is an active metabolic coenzyme and essential vitamin B6 derivative that modulates amino acid metabolism by serving as a catalytic cofactor for various PLP-dependent enzymes. It acts in transamination, decarboxylation, deamination, and racemization pathways to regulate crucial metabolic transformations. Through its essential cofactor function, pyridoxal phosphate plays a central role in biosynthesis, neurotransmitter synthesis, and cellular homeostasis. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 10 mg/mL (40.46 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 6 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.