CAS No. 41468-25-1

Batch: Purity:98
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Pyridoxal 5-phosphate monohydrate Chemical Structure
Chemical Structure
Check the Pyridoxal 5-phosphate monohydrate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 41468-25-1
Molecular Formula

C8H10NO6P.H2O
Molecular Weight (MW) 265.16
SMILES CC1=NC=C(C(=C1O)C=O)COP(=O)(O)O.O

Ordering Information

Biological Activity

Pyridoxal 5-phosphate monohydrate is an active vitamin B6 metabolite that acts as an essential enzymatic cofactor for PLP-dependent enzymes involved in amino acid metabolism. It plays a critical role in catalyzing transamination, decarboxylation, and racemization reactions within metabolic pathways. Through its participation in these enzymatic processes, pyridoxal 5-phosphate supports essential cellular functions including amino acid biosynthesis, neurotransmitter production, and central nitrogen metabolism. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 22 mg/mL (82.96 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 1 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.