CAS No. 65-22-5

Batch: Purity:99.79
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Pyridoxal hydrochloride Chemical Structure
Chemical Structure
Check the Pyridoxal hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 65-22-5
Molecular Formula

C8H10ClNO3
Molecular Weight (MW) 203.62
SMILES Cl.CC1=NC=C(CO)C(=C1O)C=O

Ordering Information

Biological Activity

Pyridoxal hydrochloride is an active endogenous metabolite and a vital form of vitamin B6 that serves as a precursor to pyridoxal 5'-phosphate. It acts as an essential enzymatic cofactor involved in amino acid metabolism, neurotransmitter biosynthesis, and cellular transamination pathways. By supporting critical metabolic enzymes, pyridoxal hydrochloride plays a key role in maintaining cellular homeostasis, regulating gene expression, and reducing oxidative stress in preclinical biological models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 40 mg/mL (196.44 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 40 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.