CAS No. 58-58-2
Batch:
Purity:99.89
Chemical Structure
Check the
Puromycin Dihydrochloride
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | (2S)-2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride | |
|---|---|---|
| CAS Number | 58-58-2 | |
| Molecular Formula |
C22H29N7O5.2HCl |
|
| Molecular Weight (MW) | 544.43 | |
| SMILES | CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl | |
| InChIKey | MKSVFGKWZLUTTO-FZFAUISWSA-N |
Biological Activity
Puromycin Dihydrochloride is an aminonucleoside antibiotic derived from Streptomyces alboniger that functions as a broad-spectrum inhibitor of protein synthesis. It acts as a structural analog of the 3' end of aminoacyl-tRNA, incorporating into the C-terminus of elongating nascent polypeptide chains to cause premature translation termination. This disruption of ribosomal translation prevents polypeptide elongation and inhibits cellular growth in prokaryotic and eukaryotic systems.
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(183.67 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 100 mg/mL |
| Ethanol | Insoluble |
| Water | 100 mg/mL |
| DMSO |
14 mg/mL
(25.71 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | Insoluble |
| DMSO |
100 mg/mL
(183.67 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 100 mg/mL |
| Ethanol | Insoluble |
| DMSO |
100 mg/mL
(183.67 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 100 mg/mL |
| Ethanol | Insoluble |
| DMSO |
100 mg/mL
(183.67 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 100 mg/mL |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Jump to
- Puromycin Dihydrochloride Mechanism of Action: Ribosome Inhibition in Mammalian Cell Research
- Puromycin Dihydrochloride Working Concentration | Cell Culture | Treatment
- Puromycin Dihydrochloride Solubility in DMSO
- Puromycin Dihydrochloride Stock Solution
- Puromycin Dihydrochloride Storage
- Puromycin Dihydrochloride Stability
- Puromycin Dihydrochloride Combination Database
- Puromycin Dihydrochloride Molecular Weight
- Puromycin Dihydrochloride SMILES
- Puromycin Dihydrochloride Chemical Structure (2D and 3D)
- Puromycin Dihydrochloride IC50 for Cell-based and Cell-free Assays
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- Puromycin Dihydrochloride SDS｜MSDS
Note: Technical data last updated: Sep 1, 2026.