CAS No. 31083-55-3

Batch: Purity:98.80
PRT4165 Chemical Structure
Chemical Structure
Check the PRT4165 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 31083-55-3
Molecular Formula

C15H9NO2

Molecular Weight (MW) 235.24
SMILES C1=CC=C2C(=C1)C(=O)C(=CC3=CN=CC=C3)C2=O

Ordering Information

Biological Activity

PRT4165 is a small-molecule inhibitor of the Bmi1/Ring1A complex that selectively targets Polycomb repressive complex 1 (PRC1) E3 ubiquitin ligase activity. By suppressing PRC1-mediated histone H2A ubiquitylation both in vitro and in cellular models, PRT4165 disrupts ubiquitin-dependent DNA damage signaling at double-strand breaks and impairs downstream Polycomb-mediated chromatin remodeling and gene repression pathways. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 11 mg/mL (46.76 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 4 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.