Procaine

For research use only.

Catalog No.S4668 Synonyms: Procaine, Novocaine, Vitamin H3, Duracaine, Spinocaine

Procaine Chemical Structure

Molecular Weight(MW): 236.31

Procaine is a benzoic acid derivative with local anesthetic and antiarrhythmic properties. Procaine binds to and inhibits voltage-gated sodium channels, thereby inhibiting the ionic flux required for the initiation and conduction of impulses.

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Biological Activity

Description Procaine is a benzoic acid derivative with local anesthetic and antiarrhythmic properties. Procaine binds to and inhibits voltage-gated sodium channels, thereby inhibiting the ionic flux required for the initiation and conduction of impulses.
In vitro

The viability of HLE, HuH7, and HuH6 cells is significantly decreased by procaine treatment. Inhibition of S/G2/M transition, morphological changes such as vacuolation and no increase in apoptosis rate are observed in the procaine-treated HLE cells. All the genes transcriptionally suppressed by DNA hypermethylation are demethylated and reactivated with procaine treatment. Procaine has growth-inhibitory and demethylating effects on human hepatoma cells[2].

In vivo Procaine has a growth-inhibitory and demethylating effect against xenograft in vivo[2].

Protocol

Cell Research:

[2]

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  • Cell lines: The human hepatoma and hepatoblastoma cell lines HLE
  • Concentrations: 1 mM
  • Incubation Time: 96 h
  • Method:

    For apoptosis analysis, the TUNEL assay is performed on HLE cells treated with no drug(control), or 1 μM of DAC(5-aza-2'-deoxycytidine), or 1 mM of PCA(procaine), or 1 mM of PCAA(procainamide) for 96 h. The proportion of TUNEL-positive cells was calculated by counting at least 500 cells randomly. All assays were carried out in triplicate.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Swiss adult albino mice
  • Dosages: 20, 30 or 50 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 47 mg/mL (198.89 mM)
Ethanol 47 mg/mL (198.89 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 236.31
Formula

C13H20N2O2

CAS No. 59-46-1
Storage powder
in solvent
Synonyms Procaine, Novocaine, Vitamin H3, Duracaine, Spinocaine
Smiles CCN(CC)CCOC(=O)C1=CC=C(N)C=C1

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID