CAS No. 501925-31-1

Batch: Purity:99.06
PNU-120596 Chemical Structure
Chemical Structure
Check the PNU-120596 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-(5-chloro-2,4-dimethoxyphenyl)-3-(5-methyl-1,2-oxazol-3-yl)urea
CAS Number 501925-31-1
Molecular Formula

C13H14ClN3O4

Molecular Weight (MW) 311.72
SMILES CC1=CC(=NO1)NC(=O)NC2=CC(=C(C=C2OC)OC)Cl
InChIKey CEIIEALEIHQDBX-UHFFFAOYSA-N

Ordering Information

Biological Activity

PNU-120596 is a selective positive allosteric modulator of α7 neuronal nicotinic acetylcholine receptors (α7 nAChR) with an EC50 of 216 nM. It exhibits no detectable activity against α4β2, α3β4, and α9α10 nicotinic receptors. Functionally, PNU-120596 enhances agonist-evoked calcium flux and increases peak currents while significantly prolonging agonist-induced channel responses, thereby potently enhancing α7 nAChR-mediated cholinergic signaling.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 62 mg/mL (198.89 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 1 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.