CAS No. 314291-83-3

Batch: Purity:99.97
PHPS1 Chemical Structure
Chemical Structure
Check the PHPS1 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-[[5-(4-nitrophenyl)-3-oxo-2-phenyl-1H-pyrazol-4-yl]diazenyl]benzenesulfonic acid
CAS Number 314291-83-3
Molecular Formula

C21H15N5O6S

Molecular Weight (MW) 465.44
SMILES O[S](=O)(=O)C1=CC=C(N\N=C2/C(=O)N(N=C2C3=CC=C(C=C3)[N+]([O-])=O)C4=CC=CC=C4)C=C1
InChIKey IYPHPQODKSHEHV-UHFFFAOYSA-N

Ordering Information

Biological Activity

PHPS1 is a potent, cell-permeable, and specific SHP2 (PTPN11) inhibitor with a Ki of. By target-inhibiting SHP2 phosphatase activity, PHPS1 blocks receptor tyrosine kinase-mediated downstream signaling, including the RAS/MAPK/ERK pathway. This inhibition attenuates growth factor-induced ERK1/2 phosphorylation, thereby suppressing cellular proliferation, cell migration, and colony formation in various cancer cell models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 93 mg/mL (199.81 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 93 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.