CAS No. 143-74-8

Batch: Purity:91.03
Phenol Red Chemical Structure
Chemical Structure
Check the Phenol Red product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 143-74-8
Molecular Formula

C19H14O5S

Molecular Weight (MW) 354.38

Ordering Information

Biological Activity

Phenol red (Phenolsulfonephthalein) is a widely used pH indicator dye that acts as a weak estrogen receptor agonist. It modulates estrogen signaling pathways, stimulating the proliferation of estrogen-responsive cells in culture and influencing cellular oxidative states through enzymatic interactions. By activating estrogen receptor-mediated gene expression, phenol red can affect cell growth, differentiation, and metabolic responses in vitro. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.