CAS No. 802539-81-7

Batch: Purity:99.79
Milciclib Chemical Structure
Chemical Structure
Check the Milciclib product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N,1,4,4-tetramethyl-8-[4-(4-methylpiperazin-1-yl)anilino]-5H-pyrazolo[4,5-h]quinazoline-3-carboxamide
CAS Number 802539-81-7
Molecular Formula

C25H32N8O

Molecular Weight (MW) 460.57
SMILES CC1(CC2=CN=C(N=C2C3=C1C(=NN3C)C(=O)NC)NC4=CC=C(C=C4)N5CCN(CC5)C)C
InChIKey RXZMYLDMFYNEIM-UHFFFAOYSA-N

Ordering Information

Biological Activity

Milciclib (PHA-848125) is an ATP-competitive cyclin-dependent kinase inhibitor targeting cyclin A/CDK2 and tropomyosin receptor kinase A (TRKA). It suppresses retinoblastoma protein phosphorylation, reduces cyclin A expression, and upregulates p21, p27, and p53 expression. These actions trigger G1 phase cell cycle arrest and induce cell death through autophagy. Additionally, Milciclib displays significant antiproliferative activity and antitumor efficacy across various preclinical carcinoma xenograft models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 17 mg/mL (36.91 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.