CAS No. 1819363-80-8

Batch: Purity:99.87
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PFI-3 Chemical Structure
Chemical Structure
Check the PFI-3 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (E)-1-(2-hydroxyphenyl)-3-[(1R,4R)-5-pyridin-2-yl-2,5-diazabicyclo[2.2.1]heptan-2-yl]prop-2-en-1-one
CAS Number 1819363-80-8
Molecular Formula

C19H19N3O2
Molecular Weight (MW) 321.37
SMILES C1C2CN(C1CN2C3=CC=CC=N3)C=CC(=O)C4=CC=CC=C4O
InChIKey INAICWLVUAKEPB-QSTFCLMHSA-N

Ordering Information

Biological Activity

PFI-3 is a selective chemical probe that targets the bromodomains of SMARCA2, SMARCA4, and polybromo 1 (PB1). By binding to these bromodomains, PFI-3 displaces SMARCA2 and SMARCA4 from chromatin, modulating SWI/SNF chromatin-remodeling complex function and downstream transcriptional pathways. In cellular studies, target displacement by PFI-3 alters chromatin accessibility without significantly compromising cancer cell proliferation or stem cell pluripotency maintenance. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 64 mg/mL (199.14 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.