CAS No. 1616287-82-1

Batch: Purity:99.02
PF-06726304 Chemical Structure
Chemical Structure
Check the PF-06726304 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 5,8-dichloro-7-(3,5-dimethyl-1,2-oxazol-4-yl)-2-[(4,6-dimethyl-2-oxo-1H-pyridin-3-yl)methyl]-3,4-dihydroisoquinolin-1-one
CAS Number 1616287-82-1
Molecular Formula

C22H21Cl2N3O3

Molecular Weight (MW) 446.33
SMILES CC1=CC(=C(C(=O)N1)CN2CCC3=C(C=C(C(=C3C2=O)Cl)C4=C(ON=C4C)C)Cl)C
InChIKey PDKDOPJQPKXNCT-UHFFFAOYSA-N

Ordering Information

Biological Activity

PF-06726304 is a potent and selective inhibitor of enhancer of zeste homolog 2 (EZH2), targeting both wild-type EZH2 and the Y641N mutant isoform. By inhibiting EZH2 methyltransferase activity, it suppresses trimethylation of histone H3 at lysine 27 (H3K27me3). Decreased H3K27me3 levels lead to derepression of silenced tumor suppressor genes, promoting cell cycle arrest, transcriptional reprogramming, and suppression of tumor cell proliferation in EZH2-dependent malignancies.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Ethanol 22 mg/mL
DMSO 10 mg/mL (22.4 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.