CAS No. 1467057-23-3
Batch:
Purity:99.44
Chemical Structure
Check the
PF-06409577
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 6-chloro-5-[4-(1-hydroxycyclobutyl)phenyl]-1H-indole-3-carboxylic acid | |
|---|---|---|
| CAS Number | 1467057-23-3 | |
| Molecular Formula |
C19H16ClNO3 |
|
| Molecular Weight (MW) | 341.79 | |
| SMILES | C1CC(C1)(C2=CC=C(C=C2)C3=C(C=C4C(=C3)C(=CN4)C(=O)O)Cl)O | |
| InChIKey | FHQXLWCFSUSXBF-UHFFFAOYSA-N |
Biological Activity
PF-06409577 is an orally bioavailable allosteric activator of AMP-activated protein kinase (AMPK), exhibiting an EC50 offor the α1β1γ1 isoform. Activation of AMPK by PF-06409577 promotes downstream phosphorylation of acetyl-CoA carboxylase, thereby regulating lipid metabolism and cellular energy homeostasis. Additionally, this compound shows no significant inhibition of hERG potassium channels or major human cytochrome P450 enzymes.
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(292.57 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 68 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Note: Technical data last updated: Sep 1, 2026.