PDGFR α Rabbit mAb

Catalog No.: F3240

Usage Information

Dilution
WB1:1000 IP1:30 IHC0.26 µg/Ml (Human, Rat) - 1:500(Mouse) IF1:500 FCM1:50

Application
WB, IP, IHC, IF, FCM, ELISA

Source
Rabbit

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
122 kDa

Datasheet & SDS

Biological Description

Specificity
PDGFR α Rabbit mAb recognizes endogenous levels of total PDGFR α protein.

Synonym(s)
PDGF Receptor alpha/PDGFR-α,PDGF Receptor α,PDGFR alpha

Clone
P1E17

Background
PDGFRα (Platelet-derived growth factor receptor alpha) is a type III receptor tyrosine kinase characterized by five extracellular immunoglobulin (Ig)-like domains, a single transmembrane domain, and an intracellular region containing a split kinase domain with a unique inserted sequence, a juxtamembrane domain, and a variable C-terminal tail. Activated by ligand-induced dimerization and autophosphorylation, PDGFRα forms homo- or heterodimers (e.g., with PDGFRβ) upon binding PDGF ligands, excluding PDGF-D. PDGFRα is predominantly expressed in cranial neural crest (CNC)-derived mesenchyme and is essential for embryonic development, particularly craniofacial and tooth morphogenesis. It regulates cell proliferation, survival, and migration in a paracrine manner, with PDGFRα expressed in odontogenic mesenchyme and its ligand PDGF-A in dental epithelium throughout all stages of tooth development. Loss of PDGFRα function results in craniofacial defects, mid-facial cleft, and embryonic lethality, highlighting its critical role in CNC-derived tissue development.

Background

PDGFRα (Platelet-derived growth factor receptor alpha) is a type III receptor tyrosine kinase characterized by five extracellular immunoglobulin (Ig)-like domains, a single transmembrane domain, and an intracellular region containing a split kinase domain with a unique inserted sequence, a juxtamembrane domain, and a variable C-terminal tail. Activated by ligand-induced dimerization and autophosphorylation, PDGFRα forms homo- or heterodimers (e.g., with PDGFRβ) upon binding PDGF ligands, excluding PDGF-D. PDGFRα is predominantly expressed in cranial neural crest (CNC)-derived mesenchyme and is essential for embryonic development, particularly craniofacial and tooth morphogenesis. It regulates cell proliferation, survival, and migration in a paracrine manner, with PDGFRα expressed in odontogenic mesenchyme and its ligand PDGF-A in dental epithelium throughout all stages of tooth development. Loss of PDGFRα function results in craniofacial defects, mid-facial cleft, and embryonic lethality, highlighting its critical role in CNC-derived tissue development.

References
[1] Heldin CH, et al. Cold Spring Harb Perspect Biol. 2013 Aug 1;5(8):a009100. [2] Xu X, et al. Dev Dyn. 2005 Jan;232(1):75-84.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%