CAS No. 614-05-1

Batch: Purity:99.00
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Oxythiamine chloride hydrochloride Chemical Structure
Chemical Structure
Check the Oxythiamine chloride hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 614-05-1
Molecular Formula

C12H16N3O2S.ClH.Cl
Molecular Weight (MW) 338.25

Biological Activity

Oxythiamine chloride hydrochloride is a thiamine antagonist and inhibitor of transketolase, a key enzyme controlling the nonoxidative branch of the pentose phosphate pathway. By suppressing transketolase activity, it impairs de novo ribose synthesis and alters metabolic flux through the pentose phosphate pathway. This disruption compromises nucleotide biosynthesis, leading to suppressed tumor cell proliferation, cell cycle arrest, and the promotion of apoptosis in cancer cells.[1][2]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 68 mg/mL (201.03 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 68 mg/mL
Ethanol 11 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Boros LG, et al. Cancer Res. 1997;57(19):4242-4248.

[2] Raïs B, et al. FEBS Lett. 1999;456(1):113-118.

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Note: Technical data last updated: Sep 1, 2026.