CAS No. 1416561-90-4
Batch:
Purity:98.18
Chemical Structure
Check the
O-Propargyl-Puromycin
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | (2S)-2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-prop-2-ynoxyphenyl)propanamide | |
|---|---|---|
| CAS Number | 1416561-90-4 | |
| Molecular Formula |
C24H29N7O5 |
|
| Molecular Weight (MW) | 495.53 | |
| InChIKey | JXBIGWQNNSJLQK-IYRMOJGWSA-N |
Biological Activity
O-Propargyl-Puromycin (OP-puro) is an alkyne puromycin analog that acts as a potent inhibitor of protein synthesis. It incorporates into nascent polypeptide chains during translation elongation on ribosomes, causing premature chain termination. This chemical tool enables the rapid labeling, detection, and quantification of newly synthesized proteins across diverse cell types and tissue explants using bioorthogonal click chemistry. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
99 mg/mL
(199.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.