CAS No. 3002056-30-3
Batch:
Purity:98.36
Chemical Structure
Check the
NST-628
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 3-[[3-fluoro-2-(methylsulfamoylamino)-4-pyridinyl]methyl]-7-[(3-fluoro-2-pyridinyl)oxy]-4-methylchromen-2-one | |
|---|---|---|
| CAS Number | 3002056-30-3 | |
| Molecular Formula |
C22H18F2N4O5S |
|
| Molecular Weight (MW) | 488.47 | |
| InChIKey | YQSZJOABXRROQR-UHFFFAOYSA-N |
Biological Activity
NST-628 is a potent pan-RAF–MEK molecular glue that targets RAF and MEK, blocking the phosphorylation and activation of MEK by RAF. By engaging all isoforms of RAF and preventing BRAF–CRAF heterodimerization, NST-628 stabilizes an inactive RAF–MEK complex. This leads to deep inhibition of downstream RAS/MAPK pathway signaling and suppresses tumor growth in diverse RAS- and RAF-driven cancer models. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
98 mg/mL
(200.62 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
Jump to
Note: Technical data last updated: Sep 1, 2026.