CAS No. 104206-65-7

Batch: Purity:99.90
NTBC (Nitisinone) Chemical Structure
Chemical Structure
Check the NTBC (Nitisinone) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-[2-nitro-4-(trifluoromethyl)benzoyl]cyclohexane-1,3-dione
CAS Number 104206-65-7
Molecular Formula

C14 H10 F3 N O5

Molecular Weight (MW) 329.23
SMILES C1CC(=O)C(C(=O)C1)C(=O)C2=C(C=C(C=C2)C(F)(F)F)[N+](=O)[O-]
InChIKey OUBCNLGXQFSTLU-UHFFFAOYSA-N

Ordering Information

Biological Activity

NTBC (Nitisinone) is a synthetic reversible inhibitor targeting 4-hydroxyphenylpyruvate dioxygenase (HPPD). By inhibiting HPPD in the tyrosine catabolic pathway, it prevents the accumulation of toxic intermediate metabolites, including succinylacetone and maleylacetoacetate. This targeted pathway blockade suppresses metabolic toxicity, preventing cell death and preserving organ function in preclinical models of hereditary tyrosinemia type 1. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 66 mg/mL (200.46 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.