CAS No. 140-40-9

Batch: Purity:99.83
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Nithiamide Chemical Structure
Chemical Structure
Check the Nithiamide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 140-40-9
Molecular Formula

C5H5N3O3S
Molecular Weight (MW) 187.18
SMILES CC(=O)NC1=NC=C(S1)[N+](=O)[O-]

Biological Activity

Nithiamide (CL 5279) is a synthetic nitroheterocyclic compound that acts as an antiprotozoal and schistosomicidal agent. As a nitrothiazole derivative, it undergoes bioreductive activation within susceptible protozoan pathogens, disrupting intracellular redox homeostasis and generating reactive intermediate species. These short-lived reactive metabolites induce oxidative damage, inhibit essential metabolic pathways, and cause cell death in microaerophilic organisms such as Trichomonas vaginalis. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 37 mg/mL (197.67 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Upcroft P, et al. Clin Microbiol Rev. 2001;14(1):150-64.

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Note: Technical data last updated: Sep 1, 2026.