CAS No. 330-95-0
Batch:
Purity:98
Chemical Structure
Check the
Nicarbazin
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 1,3-bis(4-nitrophenyl)urea;4,6-dimethyl-1H-pyrimidin-2-one | |
|---|---|---|
| CAS Number | 330-95-0 | |
| Molecular Formula |
C13H10N4O5.C6H8N2O |
|
| Molecular Weight (MW) | 426.38 | |
| SMILES | CC1=CC(=NC(=O)N1)C.C1=CC(=CC=C1NC(=O)NC2=CC=C(C=C2)[N+](=O)[O-])[N+](=O)[O-] | |
| InChIKey | UKHWDRMMMYWSFL-UHFFFAOYSA-N |
Biological Activity
Nicarbazin is an equimolar complex comprising 4,4'-dinitrocarbanilide and 2-hydroxy-4,6-dimethylpyrimidine that acts as a broad-spectrum anticoccidial and anti-parasitic agent. It exerts antiparasitic effects primarily by disrupting ATP synthesis and energy metabolism in Eimeria species, such as Eimeria tenella. This metabolic impairment inhibits second-generation schizont development, ultimately suppressing parasite replication and controlling coccidial infections in host tissues.
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
9 mg/mL
(21.1 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Note: Technical data last updated: Sep 1, 2026.