CAS No. 402-71-1
Batch:
Purity:99.33
Chemical Structure
Check the
N-p-Tosyl-L-phenylalanine chloromethyl ketone
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 402-71-1 | |
|---|---|---|
| Molecular Formula |
C17H18ClNO3S |
|
| Molecular Weight (MW) | 351.85 |
N-p-Tosyl-L-phenylalanine chloromethyl ketone (TPCK) is an irreversible inhibitor of chymotrypsin-like serine proteases. It selectively alkylates active-site histidine residues, thereby suppressing downstream signalling cascades including the NF-kB pathway by preventing IκBα degradation. Through these molecular mechanisms, TPCK modulates critical cellular functions and phenotypes, resulting in suppressed cell proliferation and tumorigenesis, as well as the induction of apoptosis. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
70 mg/mL
(198.94 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 18 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
- N-p-Tosyl-L-phenylalanine chloromethyl ketone Solubility in DMSO
- N-p-Tosyl-L-phenylalanine chloromethyl ketone Stock Solution
- N-p-Tosyl-L-phenylalanine chloromethyl ketone Storage
- N-p-Tosyl-L-phenylalanine chloromethyl ketone Stability
- N-p-Tosyl-L-phenylalanine chloromethyl ketone Molecular Weight
- N-p-Tosyl-L-phenylalanine chloromethyl ketone Chemical Structure (2D and 3D)
- N-p-Tosyl-L-phenylalanine chloromethyl ketone SDS｜MSDS
Note: Technical data last updated: Sep 1, 2026.