CAS No. 55-18-5

Batch: Purity:99.13
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N-Nitrosodiethylamine Chemical Structure
Chemical Structure
Check the N-Nitrosodiethylamine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N,N-diethylnitrous amide
CAS Number 55-18-5
Molecular Formula

C4H10N2O
Molecular Weight (MW) 102.14
SMILES CCN(CC)N=O
InChIKey WBNQDOYYEUMPFS-UHFFFAOYSA-N

Biological Activity

N-Nitrosodiethylamine is a potent DNA-reactive carcinogenic agent that requires metabolic bioactivation by cytochrome P450 enzymes, particularly CYP2E1, to form reactive alkylating intermediates. Bioactivation leads to DNA adduct formation, genotoxicity, and mutagenic events that drive tumor initiation across multiple tissues. This metabolic activation and induced genomic damage promote cell proliferation and oncogenesis, enabling its use to establish rodent cancer models, such as lung adenocarcinoma. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Verna L, et al. Pharmacol Ther. 1996;71(1-2):57-81.

[2] Mervai Z, et al. BMC Cancer. 2018;18(1):157.

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Note: Technical data last updated: Sep 1, 2026.