CAS No. 59880-97-6

Batch: Purity:99.26
Check CoA
N-Formyl-Met-Leu-Phe (fMLP) Chemical Structure
Chemical Structure
Check the N-Formyl-Met-Leu-Phe (fMLP) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2S)-2-[[(2S)-2-[[(2S)-2-formamido-4-methylsulfanylbutanoyl]amino]-4-methylpentanoyl]amino]-3-phenylpropanoic acid
CAS Number 59880-97-6
Molecular Formula

C21H31N3O5S
Molecular Weight (MW) 437.55
InChIKey PRQROPMIIGLWRP-BZSNNMDCSA-N

Ordering Information

Biological Activity

N-Formyl-Met-Leu-Phe (fMLP) is an endogenous chemotactic peptide and selective agonist of formyl peptide receptor 1 (FPR1). Activation of FPR1 by fMLP induces intracellular calcium mobilization and triggers downstream signaling through the MAPK/ERK, PI3K/AKT, and p38 MAPK pathways. These signaling cascades govern essential inflammatory cellular responses, mediating directional neutrophil migration, chemotaxis, and innate immune defense. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 88 mg/mL (201.11 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 6 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Jump to

Note: Technical data last updated: Sep 1, 2026.