CAS No. 26239-55-4

Batch: Purity:98.38
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N-(2-Acetamido)-2-Iminodiacetic acid Chemical Structure
Chemical Structure
Check the N-(2-Acetamido)-2-Iminodiacetic acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 26239-55-4
Molecular Formula

C6H10N2O5
Molecular Weight (MW) 190.15
SMILES C(C(=O)N)N(CC(=O)O)CC(=O)O

Ordering Information

Biological Activity

N-(2-Acetamido)-2-iminodiacetic acid (ADA) is a zwitterionic biological buffer component widely utilized in biochemical and biological research to maintain physiological pH within a useful range of 6.0 to 7.2. As one of Good's buffers, ADA effectively stabilizes extracellular and intracellular enzyme systems, neutralizes pH fluctuations in enzymatic assays, and serves as an important intermediate in pharmaceutical synthesis and metal-chelating applications.

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 5 mg/mL (26.29 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.