CAS No. 745017-94-1

Batch: Purity:99.94
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MMAF (Monomethylauristatin F) Chemical Structure
Chemical Structure
Check the MMAF (Monomethylauristatin F) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2S)-2-[[(2R,3R)-3-methoxy-3-[(2S)-1-[(3R,4S,5S)-3-methoxy-5-methyl-4-[methyl-[(2S)-3-methyl-2-[[(2S)-3-methyl-2-(methylamino)butanoyl]amino]butanoyl]amino]heptanoyl]pyrrolidin-2-yl]-2-methylpropanoyl]amino]-3-phenylpropanoic acid
CAS Number 745017-94-1
Molecular Formula

C39H65N5O8
Molecular Weight (MW) 731.96
SMILES CCC(C)C(C(CC(=O)N1CCCC1C(C(C)C(=O)NC(CC2=CC=CC=C2)C(=O)O)OC)OC)N(C)C(=O)C(C(C)C)NC(=O)C(C(C)C)NC
InChIKey MFRNYXJJRJQHNW-DEMKXPNLSA-N

Ordering Information

Biological Activity

MMAF (Monomethylauristatin F) is a synthetic antineoplastic agent that acts as a potent inhibitor of tubulin polymerization. By binding to tubulin, MMAF disrupts microtubule assembly, leading to cell cycle arrest at the G2/M phase and inducing apoptotic cell death. As a cytotoxic payload in antibody-drug conjugates, MMAF targetedly suppresses tumor cell proliferation. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (136.61 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 100 mg/mL
Ethanol 100 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.