CAS No. 1430213-30-1
Batch:
Purity:99.98
Chemical Structure
Check the
ML216
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 1-[4-fluoro-3-(trifluoromethyl)phenyl]-3-(5-pyridin-4-yl-1,3,4-thiadiazol-2-yl)urea | |
|---|---|---|
| CAS Number | 1430213-30-1 | |
| Molecular Formula |
C15H9F4N5OS |
|
| Molecular Weight (MW) | 383.32 | |
| SMILES | C1=CC(=C(C=C1NC(=O)NC2=NN=C(S2)C3=CC=NC=C3)C(F)(F)F)F | |
| InChIKey | WMCOYUSJXXCHFH-UHFFFAOYSA-N |
ML216 is a potent and selective inhibitor of Bloom’s syndrome protein (BLM) helicase, displaying DNA unwinding inhibition against BLM 636–1298 (IC50 = 0.97 μM) and full-length BLM (IC50 = 2.98 μM). By disrupting BLM-mediated DNA unwinding activity, ML216 impairs homologous recombination repair and genomic stability pathways. This suppression induces DNA double-strand breaks and suppresses cell proliferation, demonstrating antiproliferative and antitumor activity in cancer models. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
38 mg/mL
(99.13 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | ˂1 mg/mL |
| Ethanol | ˂1 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.