CAS No. 935273-79-3

Batch: Purity:99.33
MK-4101 Chemical Structure
Chemical Structure
Check the MK-4101 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 5-(3,3-difluorocyclobutyl)-3-[4-[4-methyl-5-[2-(trifluoromethyl)phenyl]-1,2,4-triazol-3-yl]-1-bicyclo[2.2.2]octanyl]-1,2,4-oxadiazole
CAS Number 935273-79-3
Molecular Formula

C24H24F5N5O

Molecular Weight (MW) 493.47
SMILES CN1C(=NN=C1C23CCC(CC2)(CC3)C4=NOC(=N4)C5CC(C5)(F)F)C6=CC=CC=C6C(F)(F)F
InChIKey HKJOIWLYDJCTQR-UHFFFAOYSA-N

Ordering Information

Biological Activity

MK-4101 is a potent inhibitor of the Hedgehog pathway that exhibits marked anti-tumor activity. By suppressing aberrant Hedgehog pathway activation, MK-4101 inhibits tumor cell proliferation and promotes extensive apoptosis in susceptible cell lines. Blockade of Hedgehog signaling downregulates downstream target gene expression, ultimately leading to cell cycle arrest and reduced cell viability across various cancer cell models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 98 mg/mL (198.59 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 98 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.