CAS No. 1003197-00-9
Batch:
Purity:99.53
Chemical Structure
Check the
MitoSOX Red
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 6-(3,8-diamino-6-phenyl-6H-phenanthridin-5-yl)hexyl-triphenylphosphanium iodide | |
|---|---|---|
| CAS Number | 1003197-00-9 | |
| Molecular Formula |
C43H43N3P.I |
|
| Molecular Weight (MW) | 759.70 | |
| InChIKey | NMKOJFMSKHLXRV-UHFFFAOYSA-M |
Biological Activity
MitoSOX Red is a cell-permeable fluorogenic probe designed for the selective detection of mitochondrial superoxide in living cells. Driven by its triphenylphosphonium moiety, MitoSOX Red accumulates in the mitochondrial matrix where it undergoes oxidation by superoxide to yield fluorescent products. In live cardiac myocytes and endothelial cells, MitoSOX Red detects a 3-7 fold increase in mitochondrial superoxide generation triggered by oxidative stressors, enabling the monitoring of mitochondrial redox status and ROS signaling pathways. [1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(131.63 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.