CAS No. 312608-54-1
Batch:
Purity:99.74
Chemical Structure
Check the
MID-1
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 4-ethoxy-N-(5-nitro-1,3-thiazol-2-yl)benzamide | |
|---|---|---|
| CAS Number | 312608-54-1 | |
| Molecular Formula |
C12H11N3O4S |
|
| Molecular Weight (MW) | 293.3 | |
| SMILES | CCOC1=CC=C(C=C1)C(=O)NC2=NC=C(S2)[N+]([O-])=O | |
| InChIKey | LMDLIMGAGZHZSR-UHFFFAOYSA-N |
Biological Activity
MID-1 is a small-molecule disruptor targeting the interaction between mitsugumin 53 (MG53) and insulin receptor substrate-1 (IRS-1). By disrupting the molecular association of MG53 with IRS-1, MID-1 abolishes MG53-induced IRS-1 ubiquitination and degradation in skeletal muscle. Consequently, this inhibition elevates IRS-1 expression levels, leading to enhanced downstream insulin signaling and increased glucose uptake. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
30 mg/mL
(102.28 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 1 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.