CAS No. 7413-34-5

Batch: Purity:99.00
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Methotrexate disodium Chemical Structure
Chemical Structure
Check the Methotrexate disodium product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 7413-34-5
Molecular Formula

C20H20N8O5.2Na
Molecular Weight (MW) 498.4
SMILES CN(CC1=CN=C2C(=N1)C(=NC(=N2)N)N)C3=CC=C(C=C3)C(=O)NC(CCC(=O)[O-])C(=O)[O-].[Na+].[Na+]

Ordering Information

Biological Activity

Methotrexate disodium is a synthetic folic acid analog and inhibitor of dihydrofolate reductase (DHFR). By competitively inhibiting DHFR, it disrupts intracellular folate metabolism and suppresses de novo purine and pyrimidine nucleotide synthesis, thereby impairing nucleic acid replication. This disruption of cell proliferation and metabolic homeostasis triggers programmed cell death via activation of the caspase cascade. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 99 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.