CAS No. 554-57-4
Batch:
Purity:99.67
Chemical Structure
Check the
Methazolamide
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| CAS Number | 554-57-4 | |
|---|---|---|
| Molecular Formula |
C5H8N4O3S2 |
|
| Molecular Weight (MW) | 236.27 | |
| SMILES | CC(=O)N=C1N(N=C(S1)S(=O)(=O)N)C |
Biological Activity
Methazolamide is a carbonic anhydrase inhibitor that targets hCA I, hCA II, and bCA IV. By inhibiting carbonic anhydrase enzymes, it blocks the reversible hydration of carbon dioxide, disturbing bicarbonate transport and cellular pH homeostasis. This biochemical suppression decreases aqueous humor secretion and lowers intraocular pressure in physiological systems. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
47 mg/mL
(198.92 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 3 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Reference
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Note: Technical data last updated: Sep 1, 2026.