CAS No. 231946-72-8

Batch: Purity:98.12
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LysoTracker Red(LysoTracker Red DND-99) Chemical Structure
Chemical Structure
Check the LysoTracker Red(LysoTracker Red DND-99) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 231946-72-8
Molecular Formula

C20H24BF2N5O
Molecular Weight (MW) 399.25

Biological Activity

LysoTracker Red (LysoTracker Red DND-99) is an acidotropic, red-fluorescent probe designed for selective staining and visualization of lysosomes and late endosomes in live cells. Operating as a cell-permeable weak base, it freely diffuses across biological membranes at neutral pH and accumulates within acidic organelles through protonation and charge retention. This selective intra-organellar trapping enables high-contrast imaging of lysosomal dynamics, membrane trafficking, and autophagy-related acidic compartment maturation. [1]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 80 mg/mL (200.37 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Chazotte B. Cold Spring Harb Protoc. 2011;2011(5):pdb.prot5622.

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Note: Technical data last updated: Sep 1, 2026.