CAS No. 2315-97-1
Batch:
Purity:99.22
Chemical Structure
Check the
Lucigenin
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 10-methyl-9-(10-methylacridin-10-ium-9-yl)acridin-10-ium dinitrate | |
|---|---|---|
| CAS Number | 2315-97-1 | |
| Molecular Formula |
C28H22N4O6 |
|
| Molecular Weight (MW) | 510.5 | |
| SMILES | C[N+]1=C2C=CC=CC2=C(C3=CC=CC=C31)C4=C5C=CC=CC5=[N+](C6=CC=CC=C64)C.[N+](=O)([O-])[O-].[N+](=O)([O-])[O-] | |
| InChIKey | KNJDBYZZKAZQNG-UHFFFAOYSA-N |
Lucigenin is a chemiluminescent fluorescent probe used to detect endogenously generated superoxide anion radicals in biological systems. Upon interaction with superoxide radicals, lucigenin undergoes reduction to form an acridinium intermediate that emits chemiluminescence upon oxidation, enabling sensitive detection and monitoring of reactive oxygen species production and oxidative stress pathways in vascular and cellular models. [1]
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
20 mg/mL
(39.17 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.