CAS No. 151533-22-1

Batch: Purity:99.83
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L-5-Methyltetrahydrofolate calcium Chemical Structure
Chemical Structure
Check the L-5-Methyltetrahydrofolate calcium product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 151533-22-1
Molecular Formula

C20H23N7O6.Ca
Molecular Weight (MW) 497.52
SMILES CN1C(CNC2=C1C(=O)NC(=N2)N)CNC3=CC=C(C=C3)C(=O)NC(CCC(=O)[O-])C(=O)[O-].[Ca+2]

Ordering Information

Biological Activity

L-5-Methyltetrahydrofolate calcium is a biologically active form of folate that serves as an essential cofactor and primary methyl donor in one-carbon metabolism. It participates in the remethylation of homocysteine to methionine via methionine synthase, supporting downstream DNA synthesis, repair, and epigenetic methylation pathways. This folate derivative directly bypasses enzymatic reduction steps required by synthetic folic acid, contributing to cellular nucleotide biosynthesis and amino acid homeostasis. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 1 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.