CAS No. 314245-33-5

Batch: Purity:99.86
IU1 Chemical Structure
Chemical Structure
Check the IU1 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-[1-(4-fluorophenyl)-2,5-dimethylpyrrol-3-yl]-2-pyrrolidin-1-ylethanone
CAS Number 314245-33-5
Molecular Formula

C18H21FN2O

Molecular Weight (MW) 300.37
SMILES CC1=CC(=C(N1C2=CC=C(C=C2)F)C)C(=O)CN3CCCC3
InChIKey JUWDSDKJBMFLHE-UHFFFAOYSA-N

Ordering Information

Biological Activity

IU1 is a cell-permeable, reversible, and selective inhibitor of USP14 (IC50 =, exhibiting 25-fold selectivity over IsoT. By inhibiting the deubiquitinating activity of USP14 associated with the 26S proteasome, IU1 promotes the degradation of specific proteasomal substrates and regulates cellular proteostasis. Additionally, USP14 inhibition by IU1 modulates autophagic flux and induces autophagy in various cellular models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 60 mg/mL (199.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 60 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.